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Problem 3 - Olympiad

In CRISPR-Cas9 genome editing, what is the primary role of the single-guide RNA (sgRNA)?

A. To methylate the target DNA sequenceB. To recruit the Cas9 endonuclease to the target siteC. To degrade non-specific DNA sequencesD. To initiate DNA replication at the target locus

Correct: B

The sgRNA forms a complex with Cas9 to direct it to the specific genomic target site. Methylation and replication are distinct processes, while non-specific degradation is not a CRISPR function. The Cas9 protein creates a double-strand break at the sgRNA-targeted location.