During recombinant DNA technology, which enzyme is essential for creating sticky ends in DNA to allow ligation with a vector containing complementary overhangs?
Correct: B
Restriction endonucleases like EcoRI create complementary sticky ends by cleaving DNA at specific palindromic sequences. DNA ligase joins the vector and insert DNA. Reverse transcriptase synthesizes cDNA from RNA, and DNA polymerase extends nucleotide sequences in PCR.