Which of the following vectors is most suitable for transferring large DNA fragments (>30 kb) into mammalian cells for transgenic applications?
Correct: C
BAC (Bacterial Artificial Chromosome) vectors can carry 100-300 kb of DNA fragments with low copy numbers, making them ideal for transferring large genomic DNA for transgenic studies. Cosmids have lower capacity (~45 kb) and are less efficient in mammalian systems. Plasmid vectors like pUC18 and yeast 2-micron plasmids are limited to small DNA fragments.