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Problem 20 - Entrance Test

Two independent experiments were conducted on Enzyme K. Experiment 1: Tested Enzyme K activity across a temperature range (0-100°C), finding optimal activity at 37°C and significant denaturation above 50°C. Experiment 2: Tested Enzyme K activity across a pH range (pH 2-10), finding optimal activity at pH 7.0 and drastic reduction below pH 4.0 or above pH 9.0. Which of the following conclusions is best supported by the results of both experiments regarding Enzyme K?

Correct: C

Let's analyze the findings from both experiments: - Experiment 1: Optimal at 37°C, denatures above 50°C. This indicates sensitivity to high temperatures. - Experiment 2: Optimal at pH 7.0, drastically reduced activity below pH 4.0 or above pH 9.0. This indicates sensitivity to extreme pH values (both acidic and basic). Combining these, the best supported conclusion is that Enzyme K is sensitive to both high temperatures and extreme pH values, as its activity significantly drops off outside specific ranges for both parameters.